CD22 Recombinant Rabbit Monoclonal Antibody [PO00-31]
Catalog# HA721157
CD22 Recombinant Rabbit Monoclonal Antibody [PO00-31]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD22 Recombinant Rabbit Monoclonal Antibody [PO00-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD22 aa 1-100 (N terminal).
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 95 kDa
Positive Control
Daudi (Human Burkitt's lymphoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, human B-cell lymphoma tissue, human tonsil tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
PO00-31
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
Target
Function
CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 may be a useful marker for phenotyping mature leukemias, as CD22 membrane expression has been shown to be limited to the late differentiation stages between mature B cells (CD22+) and plasma cells (CD22-). CD22 is also strongly expressed in hairy cell leukemia.
Background References
1. Shah NN. et. al. Targeting CD22 for the Treatment of B-Cell Malignancies. Immunotargets Ther. 2021 Jul
2. Dai H. et. al. Bispecific CAR-T cells targeting both CD19 and CD22 for therapy of adults with relapsed or refractory B cell acute lymphoblastic leukemia. J Hematol Oncol. 2020 Apr
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
B cell receptor CD22 precursor antibody
B lymphocyte cell adhesion molecule antibody
B-cell receptor CD22 antibody
B-lymphocyte cell adhesion molecule antibody
BL CAM antibody
BL-CAM antibody
BLCAM antibody
CD 22 antibody
CD22 antibody
CD22 antigen antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD22 on different lysates with Rabbit anti-CD22 antibody (HA721157) at 1/5,000 dilution.
Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Negative expression of CD22 protein in K-562 and Jurkat is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721157, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 95 kDa
Observed band size: 130 kDa -
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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