Placental alkaline phosphatase (PLAP) Recombinant Rabbit Monoclonal Antibody [PD00-19]
Catalog# HA721151
Placental alkaline phosphatase (PLAP) Recombinant Rabbit Monoclonal Antibody [PD00-19]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Placental alkaline phosphatase (PLAP) Recombinant Rabbit Monoclonal Antibody [PD00-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant fragment within Human Placental alkaline phosphatase (PLAP) aa 150-300.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 58 kDa
Positive Control
Human placenta tissue lysates.
Conjugation
unconjugated
Clone Number
PD00-19
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:5,000-1:10,000
Target
Function
Alkaline phosphatases are a heterogeneous group of glycoproteins confined to the cell membranes. There are three tissue-specific alkaline phosphatases; PLAP, germ cell AP (GCAP) and intestinal AP (IAP), which are 90-98% homologous with a molecular weight of 57-70 kDa. Since different antibodies may recognize PLAP alone, or PLAP together with the other AP’s and TNAP, the reaction pattern may be varying. However, in general, PLAP can be recognized consistently in seminoma/dysgerminoma/germinoma (with the exception of spermatocytic seminoma) and intratubular germ cell neoplasia (carcinoma in situ testis) as well as in most cases of embryonal carcinoma and some cases of yolk sac tumour. Hydatiform mole and choriocarcinoma are also PLAP positive. PLAP is an important marker in the identification of germ cell tumours and intratubular germ cell neoplasia. However, OCT3/4 appears to show a higher signal/noise ratio. Also PLAP has been used in the differential diagnosis of uncharacteristic tumours in, e.g., mediastinum. PLAP may also be used in the panel for the recognition of unusual neoplasms such as desmoplastic small round cell tumour, rhabdomyosarcoma and choriocarcinoma. Placenta is the best choice regarding positive control, in which the cytotrophoblasts and syncytiotrophoblasts must show an as strong as possible predominantly membranous but also cytoplasmic staining reaction, while no staining should be seen in the stromal cells. Appendix may be used as negative control, in which no structures/cells should show any staining reaction.
Background References
1. Chen J. et. al. Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP. mBio. 2020 Sep
2. Borosky GL. Alkaline Phosphatases: in Silico Study on the Catalytic Effect of Conserved Active Site Residues Using Human Placental Alkaline Phosphatase (PLAP) As a Model Protein. J Chem Inf Model. 2020 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Alkaline phosphatase antibody
Alkaline phosphatase placental antibody
Alkaline phosphatase placental type antibody
Alkaline phosphatase Regan isozyme antibody
ALP antibody
Alp1 antibody
ALPP antibody
FLJ61142 antibody
Germ-cell alkaline phosphatase antibody
nagao isozyme antibody
ExpandImages
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Western blot analysis of Placental alkaline phosphatase (PLAP) on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase (PLAP) antibody (HA721151) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 70 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721151) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Placenta
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721151, 1/10,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"