CD5 Recombinant Rabbit Monoclonal Antibody [PD00-01]
Catalog# HA721137
CD5 Recombinant Rabbit Monoclonal Antibody [PD00-01]
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IHC-P
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IF-Tissue
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WB
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Human
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unconjugated
Overview
Product Name
CD5 Recombinant Rabbit Monoclonal Antibody [PD00-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD5 aa 403 – 495 (intracellular).
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue, WB
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
Human lymph nodes tissue, human spleen tissue, Jurkat cell lysates.
Conjugation
unconjugated
Clone Number
PD00-01
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:2,000
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IF-Tissue
-
1:200
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WB
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1:5,000
Target
Function
The CD5 antigen is a 67 kDa transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increaing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthrititis and systemic lupus erythematosus. CD5 is detected in most T-cell lymphomas and leukaemias, including 75% of peripheral T-cell lymphomas and 85% of T-ALL cases. Lack of CD5 in the latter signifies a worse prognosis. Among B-cell lymphomas, more explicit CD20+ small-cell lymphomas, small lymphocytic lymphoma and mantle cell lymphoma are CD5+, whereas follicular lymphoma, marginal zone lymphoma and lymphoplasmacytoid lymphoma are CD5 negative. CD5 is detected in 5% of acute myeloid leukaemias. CD5 has been detected in some cases of thymic carcinoma and atypical thymoma. Other carcinomas are CD5 negative. Classification of small B-cell lymphomas, prognostication of T-ALL. Identification of large cell lymphoma (CD5 negative) supervening a CD5+ small lymphocytic lymphoma. Differentiation between reactive CD5+ T-cell infiltration and CD5 negative T-cell neoplasm. Identification of thymic carcinoma. Tonsil is recommendable as positive and negative tissue control, in which dispersed B-cells in the mantle zone of the secondary follicles must display a weak to moderate and distinct membranous staining reaction. T-cells will be strongly stained. No staining must be seen in the germinal center B-cells.
Background References
1. Durani U. et. al. CD5+ diffuse large B-cell lymphoma: a narrative review. Leuk Lymphoma. 2021 Dec
2. Xu Y. et. al. De Novo CD5(+) Diffuse Large B-Cell Lymphoma: Biology, Mechanism, and Treatment Advances. Clin Lymphoma Myeloma Leuk. 2020 Oct
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 5 antibody
CD5 antibody
CD5 antigen (p56 62) antibody
CD5 antigen antibody
CD5 molecule antibody
CD5_HUMAN antibody
LEU1 antibody
LyA antibody
Lymphocyte antigen T1/Leu-1 antibody
OTTHUMP00000236973 antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lymph nodes
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721137, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA721137, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: IF-Tissue
Species: Human
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
Western blot analysis of CD5 on Jurkat cell lysates with Rabbit anti-CD5 antibody (HA721137) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 67 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721137) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Primary antibody dilution: 1/2,000
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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