eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]
Catalog# HA721102
eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human eIF1A aa 71-120/144.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 16 kDa
Positive Control
Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HepG2.
Conjugation
unconjugated
Clone Number
JE64-52
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IF-Cell
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1:100
Target
Function
Eukaryotic translation initiation factor 1A, X-chromosomal (eIF1A) is a protein that in humans is encoded by the EIF1AX gene. This gene encodes an essential eukaryotic translation initiation factor. The protein is a component of the 43S pre-initiation complex (PIC), which mediates the recruitment of the small 40S ribosomal subunit to the 5' cap of messenger RNAs. eIF1A is a small protein (17 kDa in budding yeast) and a component of the 43S preinitiation complexes (PIC). eIF1A binds near the ribosomal A-site, in a manner similar to the functionally related bacterial counterpart IF1. Mutations in this gene have been recurrently seen associated to cases of uveal melanoma with disomy 3. eIF1A is mutated in thyroid cancers. EIF1AX has been shown to interact with IPO13.
Background References
1. Geng R. et. al. EIF1A depletion restrains human pituitary adenoma progression. Transl Oncol. 2022 Jan
2. Yu J. et. al. Comparative sequence and structure analysis of eIF1A and eIF1AD. BMC Struct Biol. 2018 Sep
Subcellular Location
Cytosol.
UNIPROT
Synonyms
eIF-1A antibody
eIF-1A X isoform antibody
eIF-4C antibody
EIF1A antibody
EIF1AP1 antibody
EIF1AX antibody
EIF4C antibody
eukaryotic translation initiation factor 1A X-linked antibody
eukaryotic translation initiation factor 1A, X chromosome antibody
Eukaryotic translation initiation factor 1A, X-chromosomal antibody
ExpandImages
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Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (HA721102) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721102) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling eIF1A with Rabbit anti-eIF1A antibody (HA721102) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF1A antibody (HA721102) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"