CYFIP1 Recombinant Rabbit Monoclonal Antibody [JE64-11]
Catalog# HA721095
CYFIP1 Recombinant Rabbit Monoclonal Antibody [JE64-11]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CYFIP1 Recombinant Rabbit Monoclonal Antibody [JE64-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CYFIP1 aa 51-100/1,253.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 145 kDa
Positive Control
SW480 cell lysate, rat lung tissue lysate, NIH/3T3 cell lysate, rat esophagus tissue, rat stomach tissue.
Conjugation
unconjugated
Clone Number
JE64-11
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
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1:100-1:400
Target
Function
Cytoplasmic FMR1-interacting protein 1 is a protein that in humans is encoded by the CYFIP1 gene. CYFIP1 has been shown to interact with FMR1, to the exclusion of FXR1 and FXR2. It also forms part of the WAVE regulatory complex (WRC), and based on the crystal structure of the WRC it is believed CYFIP1 provides the binding site for the complex to Rac1.This protein also interacts with the Fragile X mental retardation protein (FMRP) and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion.
Background References
1. Clifton NE. et. al. FMRP and CYFIP1 at the Synapse and Their Role in Psychiatric Vulnerability. Complex Psychiatry. 2020 Oct
2. Habela CW. et. al. Persistent Cyfip1 Expression Is Required to Maintain the Adult Subventricular Zone Neurogenic Niche. J Neurosci. 2020 Mar
Subcellular Location
Cytoplasm, perinuclear region, lamellipodium, ruffle, synaptosome.
Synonyms
CY antibody
CYFIP 1 antibody
CYFIP1 antibody
CYFP1_HUMAN antibody
Cytoplasmic FMR1 interacting protein 1 antibody
Cytoplasmic FMR1-interacting protein 1 antibody
FLJ45151 antibody
KIAA0068 antibody
p140sra 1 antibody
p140sra-1 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CYFIP1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 130 kDa
Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution.
Lane 1: SW480 (Human colorectal zdenocarcinoma cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 150 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721095, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 145.2 kDa
Observed band size: 130 kDa -
Western blot analysis of CYFIP1 on rat lung tissue lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 130 kDa
Exposure time: 30 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"