MST4 Recombinant Rabbit Monoclonal Antibody [JE64-64]
Catalog# HA721094
MST4 Recombinant Rabbit Monoclonal Antibody [JE64-64]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MST4 Recombinant Rabbit Monoclonal Antibody [JE64-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MST4 aa aa 317-416/416.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 47 kDa
Positive Control
HEK-293 cell lysate, RD cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse small intestine tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-64
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Serine/threonine protein kinase MST4, also known as mammalian STE20-like protein kinase 4 (MST-4), is a protein that in humans is encoded by the MST4 gene. The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Background References
1. An L. et. al. MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway. J Exp Med. 2020 Jun
2. Yu H. et. al. MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-beta1 Expression. Onco Targets Ther. 2020 Nov
Subcellular Location
Golgi apparatus, Cytoplasm.
Synonyms
2610018G03Rik antibody
EC 2.7.11.1 antibody
Mammalian Ste20 like protein kinase 4 antibody
Mammalian STE20-like protein kinase 4 antibody
Mammalian sterile 20 like 4 antibody
MASK antibody
MST 4 antibody
MST-4 antibody
MST3 and SOK1 related kinase antibody
Mst3 and SOK1-related kinase antibody
ExpandImages
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Western blot analysis of MST4 on different lysates with Rabbit anti-MST4 antibody (HA721094) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: RD cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Mouse small intestine tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: Lane 1-4: 51 seconds; Lane 5: 15 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721094) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"