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BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71]

Usd: 385

Specification

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Catalog# HA721091

BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human BRAT1 aa 672-821/821.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 88 kDa

Positive Control

MCF-7 cell lysate, PC-3 cell lysate, 293 cell lysate, K562 cell lysate, A431 cell lysate, THP-1 cell lysate, A549 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE64-71

RRID

AB_3072215

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:100

Target

Function

BRCA1-associated ATM activator 1 is a protein in humans that is encoded by the BRAT1 gene. The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM.

Background References

1. Scheffer IE. et. al. BRAT1 encephalopathy: a recessive cause of epilepsy of infancy with migrating focal seizures. Dev Med Child Neurol. 2020 Sep

2. Mahjoub A. et. al. Homozygous pathogenic variant in BRAT1 associated with nonprogressive cerebellar ataxia. Neurol Genet. 2019 Sep

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q6PJG6 Human

Synonyms

BAAT 1 antibody

BAAT1 antibody

BRAT 1 antibody

brat1 antibody

BRAT1_HUMAN antibody

BRCA1-associated ATM activator 1 antibody

BRCA1-associated protein required for ATM activation protein 1 antibody

BRCA1-associated protein required for ATM activation-1 antibody

C7orf27 antibody

chromosome 7 open reading frame 27 antibody

Expand

Images

  • Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (<a href="/products/HA721091" style="font-weight: bold;text-decoration: underline;">HA721091</a>) at 1/500 dilution.<br /><br />Lane 1: MCF-7 cell lysate<br />Lane 2: PC-3 cell lysate<br />Lane 3: 293 cell lysate<br />Lane 4: K562 cell lysate<br />Lane 5: A431 cell lysate<br />Lane 6: THP-1 cell lysate<br />Lane 7: A549 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 88 kDa<br />Observed band size: 77 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721091" style="font-weight: bold;text-decoration: underline;">HA721091</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (HA721091) at 1/500 dilution.

    Lane 1: MCF-7 cell lysate
    Lane 2: PC-3 cell lysate
    Lane 3: 293 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: A431 cell lysate
    Lane 6: THP-1 cell lysate
    Lane 7: A549 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 88 kDa
    Observed band size: 77 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling BRAT1 with Rabbit anti-BRAT1 antibody (<a href="/products/HA721091" style="font-weight: bold;text-decoration: underline;">HA721091</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRAT1 antibody (<a href="/products/HA721091" style="font-weight: bold;text-decoration: underline;">HA721091</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling BRAT1 with Rabbit anti-BRAT1 antibody (HA721091) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRAT1 antibody (HA721091) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"