SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]
Catalog# HA721066
SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SLC25A12 aa 601-650/678.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 75 kDa
Positive Control
Hela cell lysate, Raji cell lysate, RAW264.7 cell lysate, rat kidney tissue lysate, human kidney tissue, mouse heart tissue.
Conjugation
unconjugated
Clone Number
JE65-52
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
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IHC-P
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1:100
Target
Function
This gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in this gene may be associated with autism, and mutations in this gene may also be a cause of global cerebral hypomyelination. Alternatively spliced transcript variants have been observed for this gene. SLC25A12 (Solute Carrier Family 25 Member 12) is a Protein Coding gene. Diseases associated with SLC25A12 include Developmental And Epileptic Encephalopathy 39 and Citrullinemia, Type Ii, Adult-Onset. Among its related pathways are Glycosaminoglycan metabolism and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include calcium ion binding and L-aspartate transmembrane transporter activity. An important paralog of this gene is SLC25A13. Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.
Background References
1. Puertas-Frías G. et. al. Mitochondrial movement in Aralar/Slc25a12/AGC1 deficient cortical neurons. Neurochem Int. 2019 Dec
2. Aoki Y. et. al. Mitochondrial Aspartate/Glutamate Carrier SLC25A12 and Autism Spectrum Disorder: a Meta-Analysis. Mol Neurobiol. 2016 Apr
Subcellular Location
Mitochondrion inner membrane.
Synonyms
AGC1 antibody
Araceli hiperlarga antibody
ARALAR antibody
ARALAR1 antibody
Calcium binding mitochondrial carrier superfamily member antibody
Calcium binding mitochondrial carrier superfamily member Aralar1 antibody
Calcium-binding mitochondrial carrier protein Aralar1 antibody
CMC1_HUMAN antibody
Mitochondrial aspartate glutamate carrier 1 antibody
SLC25A12 antibody
ExpandImages
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Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (HA721066) at 1/500 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Raji cell lysate (10 µg/Lane)
Lane 3: RAW264.7 cell lysate (10 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 75 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721066) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-SLC25A12 antibody (HA721066) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721066) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"