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SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]

Usd: 385

Specification

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Catalog# HA721066

SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SLC25A12 aa 601-650/678.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 75 kDa

Positive Control

A431 cell lysate, HeLa cell lysate, RAW264.7 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human kidney tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

JE65-52

RRID

AB_3072190

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:100

Target

Function

This gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in this gene may be associated with autism, and mutations in this gene may also be a cause of global cerebral hypomyelination. Alternatively spliced transcript variants have been observed for this gene. SLC25A12 (Solute Carrier Family 25 Member 12) is a Protein Coding gene. Diseases associated with SLC25A12 include Developmental And Epileptic Encephalopathy 39 and Citrullinemia, Type Ii, Adult-Onset. Among its related pathways are Glycosaminoglycan metabolism and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include calcium ion binding and L-aspartate transmembrane transporter activity. An important paralog of this gene is SLC25A13. Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.

Background References

1. Puertas-Frías G. et. al. Mitochondrial movement in Aralar/Slc25a12/AGC1 deficient cortical neurons. Neurochem Int. 2019 Dec

2. Aoki Y. et. al. Mitochondrial Aspartate/Glutamate Carrier SLC25A12 and Autism Spectrum Disorder: a Meta-Analysis. Mol Neurobiol. 2016 Apr

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: O75746 Human

SwissProt: Q8BH59 Mouse

Entrez Gene: 362145 Rat

Synonyms

AGC1 antibody

Araceli hiperlarga antibody

ARALAR antibody

ARALAR1 antibody

Calcium binding mitochondrial carrier superfamily member antibody

Calcium binding mitochondrial carrier superfamily member Aralar1 antibody

Calcium-binding mitochondrial carrier protein Aralar1 antibody

CMC1_HUMAN antibody

Mitochondrial aspartate glutamate carrier 1 antibody

SLC25A12 antibody

Expand

Images

  • Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (<a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 80 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 74.8 kDa<br />Observed band size: 70 kDa

    Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (HA721066) at 1/1,000 dilution.

    Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 80 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721066, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 74.8 kDa
    Observed band size: 70 kDa

  • Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (<a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 74.8 kDa<br />Observed band size: 70 kDa

    Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (HA721066) at 1/1,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721066, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 74.8 kDa
    Observed band size: 70 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-SLC25A12 antibody (<a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721066" style="font-weight: bold;text-decoration: underline;">HA721066</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-SLC25A12 antibody (HA721066) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721066) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"