SSX2IP Recombinant Rabbit Monoclonal Antibody [JE65-50]
Catalog# HA721065
SSX2IP Recombinant Rabbit Monoclonal Antibody [JE65-50]
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WB
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Human
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unconjugated
Overview
Product Name
SSX2IP Recombinant Rabbit Monoclonal Antibody [JE65-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SLC25A12 aa 565-614/614.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
Daudi cell lysate, K562 cell lysate.
Conjugation
unconjugated
Clone Number
JE65-50
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
Background References
1. Chang SL. et. al. Expression and prognostic utility of SSX2IP in patients with nasopharyngeal carcinoma. APMIS. 2020 Apr
2. Reis AH. et. al. Identification of the centrosomal maturation factor SSX2IP as a Wtip-binding partner by targeted proximity biotinylation. PLoS One. 2021 Oct
Subcellular Location
Centriolar satellite, cilium basal body, nucleus, adherens junction.
UNIPROT
Synonyms
ADIP antibody
ADIP_HUMAN antibody
Afadin and alpha actinin binding protein antibody
Afadin DIL domain interacting protein antibody
Afadin DIL domain-interacting protein antibody
Afadin- and alpha-actinin-binding protein antibody
FLJ10848 antibody
KIAA0923 antibody
MGC75026 antibody
OTTHUMP00000011574 antibody
ExpandImages
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Western blot analysis of SSX2IP on different lysates with Rabbit anti-SSX2IP antibody (HA721065) at 1/500 dilution.
Lane 1: Daudi cell lysate
Lane 2: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 80/90 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721065) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"