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EF-Ts Recombinant Rabbit Monoclonal Antibody [JE64-29]

Usd: 385

Specification

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Catalog# HA721059

EF-Ts Recombinant Rabbit Monoclonal Antibody [JE64-29]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

EF-Ts Recombinant Rabbit Monoclonal Antibody [JE64-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human EF-Ts aa 1-100/325.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 35 kDa

Positive Control

HepG2 cell lysate, Jurkat cell lysate, K562 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Hela cell lysates, human kidney tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

JE64-29

RRID

AB_3072183

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:100-1:200

Target

Function

EF-Ts (elongation factor thermo stable) is one of the prokaryotic elongation factors. It is found in human mitochrondria as TSFM. It is similar to eukaryotic EF-1B. EF-Ts serves as the guanine nucleotide exchange factor for EF-Tu (elongation factor thermo unstable), catalyzing the release of guanosine diphosphate from EF-Tu. This enables EF-Tu to bind to a new guanosine triphosphate molecule, release EF-Ts, and go on to catalyze another aminoacyl tRNA addition. EF-Ts functions as guanine nucleotide exchange factor, it catalyzes the reaction of EF-Tu*GDP ( inactive form) to EF-Tu*GTP (active). EF-Tu (active) then delivers the aminoacyl-tRNA to the ribosome. Therefore, EF-Ts main role is recycling EF-Tu back to its active state in order to complete another elongation cycle.

Background References

1. Scala M. et. al. Novel homozygous TSFM pathogenic variant associated with encephalocardiomyopathy with sensorineural hearing loss and peculiar neuroradiologic findings. Neurogenetics. 2019 Aug

2. Traschütz A. et. al. TSFM mutations cause a complex hyperkinetic movement disorder with strong relief by cannabinoids. Parkinsonism Relat Disord. 2019 Mar

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: P43897 Human

SwissProt: Q9CZR8 Mouse

Synonyms

COXPD 3 antibody

COXPD3 antibody

EF TS antibody

EF Tsmt antibody

EF-Ts antibody

EF-TsMt antibody

EFTS antibody

EFTS_HUMAN antibody

EFTSMT antibody

Elongation factor Ts antibody

Expand

Images

  • Western blot analysis of EF-Ts on different lysates with Rabbit anti-EF-Ts antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/500 dilution.<br /><br />Lane 1: HepG2 cell lysate, 10 µg/Lane<br />Lane 2: Jurkat cell lysate, 10 µg/Lane<br />Lane 3: K562 cell lysate, 10 µg/Lane<br />Lane 4: Mouse liver tissue lysate, 20 µg/Lane<br />Lane 5: Mouse kidney tissue lysate, 20 µg/Lane<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 1 minute;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EF-Ts on different lysates with Rabbit anti-EF-Ts antibody (HA721059) at 1/500 dilution.

    Lane 1: HepG2 cell lysate, 10 µg/Lane
    Lane 2: Jurkat cell lysate, 10 µg/Lane
    Lane 3: K562 cell lysate, 10 µg/Lane
    Lane 4: Mouse liver tissue lysate, 20 µg/Lane
    Lane 5: Mouse kidney tissue lysate, 20 µg/Lane

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 1 minute;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721059) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of EF-Ts on Hela cell lysates with Rabbit anti-EF-Ts antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 1 minute;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EF-Ts on Hela cell lysates with Rabbit anti-EF-Ts antibody (HA721059) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 1 minute;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721059) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EF-Ts antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EF-Ts antibody (HA721059) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721059) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-EF-Ts antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721059" style="font-weight: bold;text-decoration: underline;">HA721059</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-EF-Ts antibody (HA721059) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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