UFD1L Recombinant Rabbit Monoclonal Antibody [JE64-80]
Catalog# HA721057
UFD1L Recombinant Rabbit Monoclonal Antibody [JE64-80]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
UFD1L Recombinant Rabbit Monoclonal Antibody [JE64-80]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human UFD1L aa 11-60/307.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
MCF7 cell lysate, MOLT-4 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-80
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
Background References
1. Yan J. et. al. Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species. Virus Res. 2020 Jul
2. Huiting LN. et. al. UFD1 contributes to MYC-mediated leukemia aggressiveness through suppression of the proapoptotic unfolded protein response. Leukemia. 2018 Nov
Subcellular Location
Nucleus, cytosol.
Synonyms
UB fusion protein 1 antibody
Ubiquitin fusion degradation 1 like (yeast) antibody
Ubiquitin fusion degradation 1 like antibody
Ubiquitin fusion degradation protein 1 homolog antibody
UFD1 antibody
UFD1_HUMAN antibody
UFD1L antibody
Images
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Western blot analysis of UFD1L on different lysates with Rabbit anti-UFD1L antibody (HA721057) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate (10 µg/Lane)
Lane 2: MOLT-4 cell lysate (10 µg/Lane)
Lane 3: PC-12 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 39 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721057) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"