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WDR1 Recombinant Rabbit Monoclonal Antibody [JE64-32]

Usd: 385

Specification

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Catalog# HA721053

WDR1 Recombinant Rabbit Monoclonal Antibody [JE64-32]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

WDR1 Recombinant Rabbit Monoclonal Antibody [JE64-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human WDR1 aa 301-350/606.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 66 kDa

Positive Control

HeLa cell lysate, Raji cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat skeletal muscle tissue lysate, human gastric tissue, human endometrial tissue.

Conjugation

unconjugated

Clone Number

JE64-32

RRID

AB_3072177

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:100-1:400

Target

Function

WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene.This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Kuhns D.B. et. al. Cytoskeletal abnormalities and neutrophil dysfunction in WDR1 deficiency. Blood 128:2135-2143(2016)

2. Pfajfer L. et. al. Mutations affecting the actin regulator WD repeat-containing protein 1 lead to aberrant lymphoid immunity. J. Allergy Clin. Immunol. 142:1589-1604(2018)

Subcellular Location

Cytoskeleton, Cytoplasm, podosome, Cell junction.

UNIPROT

SwissProt: O75083 Human

SwissProt: O88342 Mouse

SwissProt: Q5RKI0 Rat

Synonyms

Actin interacting protein 1 antibody

Actin-interacting protein 1 antibody

AIP1 antibody

D5Wsu185e antibody

NORI 1 antibody

NORI-1 antibody

WD repeat domain 1 antibody

WD repeat-containing protein 1 antibody

wdr1 antibody

WDR1_HUMAN antibody

Images

  • Western blot analysis of WDR1 on different lysates with Rabbit anti-WDR1 antibody (<a href="/products/HA721053" style="font-weight: bold;text-decoration: underline;">HA721053</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: Rat skeletal muscle tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: Lane 1-4: 23 seconds; Lane 5: 1 minute 7 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721053" style="font-weight: bold;text-decoration: underline;">HA721053</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of WDR1 on different lysates with Rabbit anti-WDR1 antibody (HA721053) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Raji cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: C6 cell lysate
    Lane 5: Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 75 kDa

    Exposure time: Lane 1-4: 23 seconds; Lane 5: 1 minute 7 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human gastric tissue with Rabbit anti-WDR1 antibody at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody WDR1 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human gastric tissue with Rabbit anti-WDR1 antibody at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody WDR1 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human endometrial tissue with Rabbit anti-WDR1 antibody at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody WDR1 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrial tissue with Rabbit anti-WDR1 antibody at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody WDR1 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"