IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-54]
Catalog# HA721041
IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-54]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IQGAP2 aa 1,301-1,500/1,575.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 181 kDa
Positive Control
Human placenta tissue lysates, human gallbladder tissue, human prostate tissue.
Conjugation
unconjugated
Clone Number
JE64-54
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
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IHC-P
-
1:100
Target
Function
This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants.
Background References
1. de Ligt J., et al. Diagnostic exome sequencing in persons with severe intellectual disability. N. Engl. J. Med. 367:1921-1929(2012).
2. Kumar D, et al. Reduced expression of IQGAP2 and higher expression of IQGAP3 correlates with poor prognosis in cancers. PLoS One. 2017 Oct 26;12(10):e0186977.
Subcellular Location
Actin cytoskeleton, microtubule, cytosol, extracellular exosome, plasma membrane, cell surface, cytoplasm, filopodium, lamellipodium, microvillus, secretory granule membrane
UNIPROT
Synonyms
IQ motif containing GTPase activating protein 2 antibody
IQGA2_HUMAN antibody
Iqgap2 antibody
Ras GTPase activating-like protein antibody
Ras GTPase-activating-like protein IQGAP2 antibody
Images
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Western blot analysis of IQGAP2 on human placenta tissue lysates with Rabbit anti-IQGAP2 antibody (HA721041) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 181 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721041) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of IQGAP2 on different lysates with Rabbit anti-IQGAP2 antibody (HA721041) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-IQGAP2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 181 kDa
Exposure time: 120 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721041) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (HA721041) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721041) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-IQGAP2 antibody (HA721041) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721041) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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