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AdSS 2 Recombinant Rabbit Monoclonal Antibody [JE64-42]

Usd: 385

Specification

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Catalog# HA721040

AdSS 2 Recombinant Rabbit Monoclonal Antibody [JE64-42]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

AdSS 2 Recombinant Rabbit Monoclonal Antibody [JE64-42]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human AdSS 2 aa 1-200/456.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 50 kDa

Positive Control

293T cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

JE64-42

RRID

AB_3072164

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP. This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.

Background References

1. Li X, et al. Comparative molecular characterization of ADSS1 and ADSS2 genes in pig (Sus scrofa). Comp Biochem Physiol B Biochem Mol Biol. 2007 Jun;147(2):271-7.

2. Xia Y, et al. Electrical stimulation of neonatal cardiac myocytes activates the NFAT3 and GATA4 pathways and up-regulates the adenylosuccinate synthetase 1 gene. J Biol Chem. 2000 Jan 21;275(3):1855-63.

Subcellular Location

Cytoplasm, Mitochondrion.

UNIPROT

SwissProt: P30520 Human

SwissProt: P46664 Mouse

SwissProt: D4AEP0 Rat

Synonyms

(Ade(-)H, complement of antibody

acidic isozyme antibody

ADEH antibody

Adenylosuccinate synthetase (Ade(-)H complementing) antibody

Adenylosuccinate synthetase acidic isozyme antibody

Adenylosuccinate synthetase antibody

Adenylosuccinate synthetase isozyme 2 antibody

Adenylosuccinate synthetase liver isozyme antibody

AdSS 2 antibody

ADSS antibody

Expand

Images

  • Western blot analysis of AdSS 2 on different lysates with Rabbit anti-AdSS 2 antibody (<a href="/products/HA721040" style="font-weight: bold;text-decoration: underline;">HA721040</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: HL-60 cell lysate (20 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721040" style="font-weight: bold;text-decoration: underline;">HA721040</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AdSS 2 on different lysates with Rabbit anti-AdSS 2 antibody (HA721040) at 1/1,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: HL-60 cell lysate (20 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 4: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721040) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"