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Cip4 Recombinant Rabbit Monoclonal Antibody [JE63-45]

Usd: 385

Specification

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Catalog# HA721029

Cip4 Recombinant Rabbit Monoclonal Antibody [JE63-45]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cip4 Recombinant Rabbit Monoclonal Antibody [JE63-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cip4 aa 1-50/601.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 68 kDa

Positive Control

JAR cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysate, PC-12 cell lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE63-45

RRID

AB_3072153

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.

Background References

1. Tonucci FM. et. al. Identification of a CIP4 PKA phosphorylation site involved in the regulation of cancer cell invasiveness and metastasis. Cancer Lett. 2019 Oct

2. Truesdell P. et. al. CIP4 promotes lung adenocarcinoma metastasis and is associated with poor prognosis. Oncogene. 2015 Jul

Subcellular Location

Cell membrane, lysosome, cytoskeleton, golgi apparatus, cell cortex, phagocytic cup; perinuclear region.

UNIPROT

SwissProt: Q15642 Human

SwissProt: Q8CJ53 Mouse

SwissProt: P97531 Rat

Synonyms

Cdc42 interacting protein 4 antibody

Cdc42 interaction protein 4 long isoform antibody

Cdc42-interacting protein 4 antibody

CG11341 antibody

CG15015 PA antibody

Cip 4 antibody

CIP4 antibody

CIP4_HUMAN antibody

DCIP4 antibody

hSTP antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (<a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Cip4 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Cip4 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 80 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (<a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate, 10 µg/Lane<br />Lane 2: Rat brain tissue lysate, 20 µg/Lane<br /><br />Predicted band size: 68 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate, 10 µg/Lane
    Lane 2: Rat brain tissue lysate, 20 µg/Lane

    Predicted band size: 68 kDa
    Observed band size: 75 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (<a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>) at 1/1,000 dilution.<br /><br />Lane 1: JAR （Human placental villus cancer cell) cell lysate<br />Lane 2: 293T (Human embryonic kidney cell) cell lysate<br />Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721029" style="font-weight: bold;text-decoration: underline;">HA721029</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 68 kDa<br />Observed band size: 80 kDa

    Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution.

    Lane 1: JAR （Human placental villus cancer cell) cell lysate
    Lane 2: 293T (Human embryonic kidney cell) cell lysate
    Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721029, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 68 kDa
    Observed band size: 80 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"