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SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]

Usd: 385

Specification

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Catalog# HA721023

SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]

Application

  • WB

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SARA aa 1-50/1,425.

Species Reactivity

Human, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 156 kDa

Positive Control

HUVEC cell lysate, HeLa cell lysate, SiHa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE63-65

RRID

AB_3072147

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants.

Background References

1. Wojtowicz S. et. al. SMURF2 and SMAD7 induce SARA degradation via the proteasome. Cell Signal. 2020 Aug

2. Rozés-Salvador V. et. al. Fine-Tuning the TGFbeta Signaling Pathway by SARA During Neuronal Development. Front Cell Dev Biol. 2020 Sep

Subcellular Location

Early endosome membrane, Cytoplasm.

UNIPROT

SwissProt: O95405 Human

Entrez Gene: 313477 Rat

Synonyms

CG15667 antibody

hSARA antibody

LD33044p antibody

Madh-interacting protein antibody

MADHIP antibody

Mothers against decapentaplegic homolog-interacting protein antibody

Novel serine protease antibody

NSP antibody

Receptor activation anchor antibody

SAR1 antibody

Expand

Images

  • Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (<a href="/products/HA721023" style="font-weight: bold;text-decoration: underline;">HA721023</a>) at 1/1,000 dilution.<br /><br />Lane 1: HUVEC cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: SiHa cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 156 kDa<br />Observed band size: 156 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721023" style="font-weight: bold;text-decoration: underline;">HA721023</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution.

    Lane 1: HUVEC cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: SiHa cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 156 kDa
    Observed band size: 156 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (<a href="/products/HA721023" style="font-weight: bold;text-decoration: underline;">HA721023</a>) at 1/500 dilution.<br /><br />Lane 1: HUVEC cell lysate, 10 µg/Lane<br />Lane 2: Rat brain tissue lysate, 20 µg/Lane<br /><br />Predicted band size: 156 kDa<br />Observed band size: 156 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721023" style="font-weight: bold;text-decoration: underline;">HA721023</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/500 dilution.

    Lane 1: HUVEC cell lysate, 10 µg/Lane
    Lane 2: Rat brain tissue lysate, 20 µg/Lane

    Predicted band size: 156 kDa
    Observed band size: 156 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"