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Phospho-MCM2 (S108) Recombinant Rabbit Monoclonal Antibody [JE63-52]

Usd: 385

Specification

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Catalog# HA721021

Phospho-MCM2 (S108) Recombinant Rabbit Monoclonal Antibody [JE63-52]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-MCM2 (S108) Recombinant Rabbit Monoclonal Antibody [JE63-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within corresponding to residues surrounding ser108 of Human MCM2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 102 kDa

Positive Control

SiHa cell lysate, HL-60 cell lysate, HeLa cell lysate, RAW264.7 cell lysate, C6 cell lysate, human lymph node tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

JE63-52

RRID

AB_3072145

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:9,000

Target

Function

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

Background References

1. Issac MSM. et. al. MCM2, MCM4, and MCM6 in Breast Cancer: Clinical Utility in Diagnosis and Prognosis. Neoplasia. 2019 Oct

2. Yang WX. et. al. CDK1, CCNB1, CDC20, BUB1, MAD2L1, MCM3, BUB1B, MCM2, and RFC4 May Be Potential Therapeutic Targets for Hepatocellular Carcinoma Using Integrated Bioinformatic Analysis. Biomed Res Int. 2019 Oct

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P49736 Human

SwissProt: P97310 Mouse

Entrez Gene: 312538 Rat

Synonyms

BM28 antibody

CCNL 1 antibody

CCNL1 antibody

CDC like 1 antibody

cdc19 antibody

CDCL 1 antibody

CDCL1 antibody

Cell devision cycle like 1 antibody

Cyclin like 1 antibody

D3S3194 antibody

Expand

Images

  • Western blot analysis of Phospho-MCM2 (S108) on different lysates with Rabbit anti-Phospho-MCM2 (S108) antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/5,000 dilution.<br /><br />Lane 1: SiHa cell lysate<br />Lane 2: HL-60 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: HL-60 cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 125 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-MCM2 (S108) on different lysates with Rabbit anti-Phospho-MCM2 (S108) antibody (HA721021) at 1/5,000 dilution.

    Lane 1: SiHa cell lysate
    Lane 2: HL-60 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: HL-60 cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 125 kDa

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721021) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (HA721021) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721021) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/9,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (HA721021) at 1/9,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721021) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/9,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721021" style="font-weight: bold;text-decoration: underline;">HA721021</a>) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Phospho-MCM2 (S108) antibody (HA721021) at 1/9,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721021) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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