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SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]

Usd: 385

Specification

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Catalog# HA721018

SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SLC45A3 aa 400-500.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 59 kDa

Positive Control

Human prostate tissue, human prostate cancer tissue.

Conjugation

unconjugated

Clone Number

JE58-86

RRID

AB_3072142

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:200

Target

Function

Solute carrier family 45 member 3 (SLC45A3), also known as prostate cancer-associated protein 6 or prostein, is a protein that in humans is encoded by the SLC45A3 gene. SLC45A3 is an androgen-regulated gene and a prostate specific marker expressed in prostatic glandular cells. SLC45A3 is the second most common gene fusion partner in prostate cancers. SLC45A3-ERG and SLC45A3-ELK4 fusion transcripts have been detected in prostate cancer. Loss of prostein expression in SLC45A3-ERG tumors are significantly associated with high Gleason scores and poor clinical outcomes. Prostein immunoreactivity is seen in the majority of primary and metastatic prostate carcinomas. Staining for prostein in conjunction with PSA improves identification of prostatic origin in unknown primary lesions, when compared against prostein or PSA staining alone.

Background References

1. Qin F. et. al. SLC45A3-ELK4 functions as a long non-coding chimeric RNA. Cancer Lett. 2017 Sep

2. Hernández-Llodrà S. et. al. ERG overexpression plus SLC45A3 (prostein) and PTEN expression loss: Strong association of the triple hit phenotype with an aggressive pathway of prostate cancer progression. Oncotarget. 2017 May

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q96JT2 Human

Synonyms

IPCA 2 antibody

IPCA 8 antibody

IPCA6 antibody

PCANAP2 antibody

PCANAP6 antibody

PCANAP8 antibody

Prostate cancer associated gene 2 antibody

Prostate cancer associated gene 6 antibody

Prostate cancer associated gene 8 antibody

Prostate cancer associated protein 2 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721018" style="font-weight: bold;text-decoration: underline;">HA721018</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721018" style="font-weight: bold;text-decoration: underline;">HA721018</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: prostate cancer<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: prostate cancer

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"