FITC Conjugated Human CD33 Recombinant Rabbit Monoclonal Antibody [PSH04-96]
Catalog# HA720197F
FITC Conjugated Human CD33 Recombinant Rabbit Monoclonal Antibody [PSH04-96]
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FC
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Human
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FITC
Overview
Product Name
FITC Conjugated Human CD33 Recombinant Rabbit Monoclonal Antibody [PSH04-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD33 aa 1-282 / 364 (P20138).
Species Reactivity
Human
Validated Applications
FC
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
Human peripheral blood.
Conjugation
FITC
Clone Number
PSH04-96
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| FC | |
|---|---|
| human |
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Product Features
Form
Liquid
Concentration
/(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Target
Function
CD33 or Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67) is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. It binds sialic acids, therefore is a member of the SIGLEC family of lectins. CD33 can be stimulated by any molecule with sialic acid residues such as glycoproteins or glycolipids. Upon binding, the immunoreceptor tyrosine-based inhibition motif (ITIM) of CD33, present on the cytosolic portion of the protein, is phosphorylated and acts as a docking site for Src homology 2 (SH2) domain-containing proteins like SHP phosphatases. This results in a cascade that inhibits phagocytosis in the cell.
Background References
1. Albinger N et al. Primary CD33-targeting CAR-NK cells for the treatment of acute myeloid leukemia. Blood Cancer J. 2022 Apr
2. Willier S et al. CLEC12A and CD33 coexpression as a preferential target for pediatric AML combinatorial immunotherapy. Blood. 2021 Feb
Subcellular Location
Cell membrane; Peroxisome.
UNIPROT
Synonyms
CD 33 antibody
CD33 antibody
CD33 antigen (gp67) antibody
CD33 antigen antibody
CD33 molecule antibody
CD33_HUMAN antibody
FLJ00391 antibody
gp67 antibody
My9 antibody
Myeloid cell surface antigen CD33 antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood labelling Human CD33 (HA720197F, FITC).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"