iFluor™ 594 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287] (HA720164F)
Catalog# HA720164F
Rabbit Monoclonal to E-Cadherin
Tested for
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IF-Tissue
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 594
Immunofluorescence analysis of paraffin-embedded human breast carcinoma tissue labeling E-Cadherin (HA720164F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody E-Cadherin (HA720164F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody E-Cadherin (HA720164F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.