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iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]

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Specification

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Catalog# HA720141F

iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]

Application

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • iFluor™ 647

Overview

Product Name

iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within mouse Cytokeratin 1 aa 586-637.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IF-Tissue

Molecular Weight

Predicted band size: 66 kDa

Positive Control

Human skin tissue.

Conjugation

iFluor™ 647

Clone Number

SN72-08

RRID

AB_3072080

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Tissue

  • 1:100-1:400

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.

Background References

1. Zhu, H. et al. 2016. MiR-217 is down-regulated in psoriasis and promotes keratinocyte differentiation via targeting GRHL2. Biochemical and biophysical research communications. 471: 169-76.

2. Li, G. et al. 2015. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. The Journal of biological chemistry. 290: 20448-54.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P04264 Human

SwissProt: P04104 Mouse

SwissProt: Q6IMF3 Rat

Synonyms

67 kDa cytokeratin antibody

CK-1 antibody

CK1 antibody

Cytokeratin-1 antibody

Cytokeratin1 antibody

EHK antibody

EHK1 antibody

Epidermolytic hyperkeratosis 1 antibody

EPPK antibody

Hair alpha protein antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (<a href="/products/HA720141F" style="font-weight: bold;text-decoration: underline;">HA720141F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (<a href="/products/HA720141F" style="font-weight: bold;text-decoration: underline;">HA720141F</a>, red) at 1/100 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />iFluor&trade; 488 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (<a href="/products/HA720141F" style="font-weight: bold;text-decoration: underline;">HA720141F</a>), Cytokeratin 5 (<a href="/products/HA720129F" style="font-weight: bold;text-decoration: underline;">HA720129F</a>) and Cytokeratin 10 (<a href="/products/HA720119F" style="font-weight: bold;text-decoration: underline;">HA720119F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (<a href="/products/HA720141F" style="font-weight: bold;text-decoration: underline;">HA720141F</a>, red) at 1/400 dilution, Cytokeratin 5 (<a href="/products/HA720129F" style="font-weight: bold;text-decoration: underline;">HA720129F</a>, green) at 1/100 dilution and Cytokeratin 10 (<a href="/products/HA720119F" style="font-weight: bold;text-decoration: underline;">HA720119F</a>, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS.<br /><br />DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F), Cytokeratin 5 (HA720129F) and Cytokeratin 10 (HA720119F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS.

    DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"