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iFluor™ 488 Conjugated Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]

Usd: 429

Specification

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Catalog# HA720140F

iFluor™ 488 Conjugated Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]

Application

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • iFluor™ 488

Overview

Product Name

iFluor™ 488 Conjugated Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 19 aa 348-400.

Species Reactivity

Human, Mouse

Validated Applications

IF-Cell, IF-Tissue, FC

Molecular Weight

Predicted band size: 44 kDa

Positive Control

MCF-7, SK-Br-3, human breast tissue.

Conjugation

iFluor™ 488

Clone Number

SA30-06

RRID

AB_3072079

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:100

  • FC

  • 1ug/mL

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers.

Background References

1. Guye P. et. al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016).

2. Cui M. et. al. PTEN is a potent suppressor of small cell lung cancer. Mol Cancer Res 12:654-9 (2014).

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P08727 Human

SwissProt: P19001 Mouse

Synonyms

40 kDa keratin intermediate filament antibody

CK 19 antibody

CK-19 antibody

CK19 antibody

Cytokeratin 19 antibody

Cytokeratin-19 antibody

K19 antibody

K1C19_HUMAN antibody

K1CS antibody

Keratin 19 antibody

Expand

Images

  • Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 19 with Rabbit anti-Cytokeratin 19 antibody (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 19 antibody (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) were used as the secondary antibody at 1/800 dilution.

    Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 19 with Rabbit anti-Cytokeratin 19 antibody (HA720140F) at 1/100 dilution.

    Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 19 antibody (HA720140F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  • Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19 with Rabbit anti-Cytokeratin 19 antibody (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>) at 1/50 dilution.<br /><br />Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 19 antibody (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19 with Rabbit anti-Cytokeratin 19 antibody (HA720140F) at 1/50 dilution.

    Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 19 antibody (HA720140F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 19 (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>, iFluor&trade; 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 19 (HA720140F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720140F, iFluor™ 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 19.<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (<a href="/products/HA720140F" style="font-weight: bold;text-decoration: underline;">HA720140F</a>, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 19.

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720140F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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