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iFluor™ 488 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Usd: 429

Specification

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Catalog# HA720131F

iFluor™ 488 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Application

  • IF-Tissue

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • iFluor™ 488

Overview

Product Name

iFluor™ 488 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 7 aa 18-67.

Species Reactivity

Human, Mouse

Validated Applications

IF-Tissue, IF-Cell, FC

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

Human liver tissue, A431, human breast tissue, SK-Br-3.

Conjugation

iFluor™ 488

Clone Number

ST50-05

RRID

AB_3072070

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Tissue

  • 1:200

  • IF-Cell

  • 1:100

  • FC

  • 1:500-1:1,000

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

Background References

1. Hrudka J. et al. Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma. Sci Rep. 2021 Sep

2. Statz E. et al. Cytokeratin 7, GATA3, and SOX-10 is a Comprehensive Panel in Diagnosing Triple Negative Breast Cancer Brain Metastases. Int J Surg Pathol. 2021 Aug

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P08729 Human

Synonyms

CK 7 antibody

CK-7 antibody

CK7 antibody

Cytokeratin 7 antibody

Cytokeratin-7 antibody

D15Wsu77e antibody

K2C7 antibody

K2C7_HUMAN antibody

K7 antibody

Keratin 7 antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>, green) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />iFluor&trade; 594 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720131F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720131F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

  • Immunocytochemistry analysis of A431 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) were used as the secondary antibody at 1/800 dilution.

    Immunocytochemistry analysis of A431 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (HA720131F) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (HA720131F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>, iFluor&trade; 488) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720131F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720131F, iFluor™ 488) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (<a href="/products/HA720131F" style="font-weight: bold;text-decoration: underline;">HA720131F</a>, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720131F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"