iFluor™ 594 Conjugated Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody [SN74-03]
Catalog# HA720123F
iFluor™ 594 Conjugated Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody [SN74-03]
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IF-Tissue
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Human
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody [SN74-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Cytokeratin 4 aa 395-520.
Species Reactivity
Human
Validated Applications
IF-Tissue
Molecular Weight
57 kDa
Positive Control
Human tonsil tissue.
Conjugation
iFluor™ 594
Clone Number
SN74-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:100
Target
Function
Keratin, type I cytoskeletal 4 also known as cytokeratin-4 (CK-4) or keratin-4 (K4) is a protein that in humans is encoded by the KRT4 gene.Keratin 4 is a type II cytokeratin. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13. Mutations in the genes encoding this protein have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia.
Background References
1. Jacot TA et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014).
2. Badylak SF et al. Esophageal preservation in five male patients after endoscopic inner-layer circumferential resection in the setting of superficial cancer: a regenerative medicine approach with a biologic scaffold. Tissue Eng Part A 17:1643-50 (2011).
Subcellular Location
Cell surface, intermediate filament cytoskeleton, nucleus.
UNIPROT
Synonyms
CK 4 antibody
CK-4 antibody
CK4 antibody
CYK4 antibody
Cytokeratin 4 antibody
Cytokeratin-4 antibody
Cytokeratin4 antibody
FLJ31692 antibody
K2C4_HUMAN antibody
K4 antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 4 (HA720123F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 4 (HA720123F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"