iFluor™ 594 Conjugated Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06]
Catalog# HA720117F
iFluor™ 594 Conjugated Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06]
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IF-Tissue
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Rat
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Human
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Mouse
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Cytokeratin 14 aa 250-484.
Species Reactivity
Rat (Predicted: Human, Mouse)
Predicted species support after-sales service
Validated Applications
IF-Tissue
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Rat skin tissue.
Conjugation
iFluor™ 594
Clone Number
SC65-06
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:100-1:500
Target
Function
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. A form of epidermolysis bullosa simplex, a group of skin fragility disorders characterized by skin blistering due to cleavage within the basal layer of keratinocytes, and erosions caused by minor mechanical trauma. There is a broad spectrum of clinical severity ranging from minor blistering on the feet, to subtypes with extracutaneous involvement and a lethal outcome. EBS1A is an autosomal dominant form characterized by generalized intraepidermal skin blistering that begins and is very prominent at birth. EBS1A may be life-threatening in the first year of life. Tendency to blistering diminishes in adolescence.
Background References
1. Pastar I et al. Interactions of methicillin resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in polymicrobial wound infection. PLoS One 8:e56846 (2013).
2. DeWard AD et al. Cellular heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population. Cell Rep 9:701-11 (2014).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
CK 14 antibody
CK-14 antibody
ck14 antibody
Cytokeratin 14 antibody
Cytokeratin-14 antibody
Cytokeratin14 antibody
Dowling Meara antibody
EBS3 antibody
EBS4 antibody
Epidermolysis bullosa simplex antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (HA720117F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (HA720117F, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (HA720117F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (HA720117F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"