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TRPV5 Recombinant Rabbit Monoclonal Antibody [JE62-37]

Usd: 385

Specification

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Catalog# HA720109

TRPV5 Recombinant Rabbit Monoclonal Antibody [JE62-37]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TRPV5 Recombinant Rabbit Monoclonal Antibody [JE62-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TRPV5 aa 1-150/729.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 83 kDa

Positive Control

SiHa cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, rat kidney tissue lysates.

Conjugation

unconjugated

Clone Number

JE62-37

RRID

AB_3072048

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Transient receptor potential cation channel subfamily V member 5 is a calcium channel protein that in humans is encoded by the TRPV5 gene. The TRPV5 gene is a member of the transient receptor family and the TRPV subfamily. The calcium-selective channel, TRPV5, encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. Both TRPV5 and TRPV6 are expressed in kidney and intestinal epithelial cells. TRPV5 is mainly expressed in kidney epithelial cells, where it plays an important role in the reabsorption of Ca2+, whereas TRPV6 is mainly expressed in the intestine. The enzyme α-klotho increases kidney calcium reabsorption by stabilizing TPRV5. Klotho is a beta-glucuronidase-like enzyme that activates TRPV5 by removal of sialic acid.

Background References

1. Hughes TE.et. al. Structure-based characterization of novel TRPV5 inhibitors. Elife. 2019 Oct

2. van der Wijst J.et. al. TRPV5 in renal tubular calcium handling and its potential relevance for nephrolithiasis. Kidney Int. 2019 Dec

Subcellular Location

Apical cell membrane.

UNIPROT

SwissProt: Q9NQA5 Human

SwissProt: P69744 Mouse

SwissProt: Q9JIP0 Rat

Synonyms

Calcium transport protein 2 antibody

Calcium transporter 2 antibody

CAT 2 antibody

CAT2 antibody

ECAC 1 antibody

ECaC antibody

ECaC1 antibody

Epithelial calcium channel 1 antibody

Osm 9 like TRP channel 3 antibody

Osm-9-like TRP channel 3 antibody

Expand

Images

  • Western blot analysis of TRPV5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA720109" style="font-weight: bold;text-decoration: underline;">HA720109</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: SiHa cell lysate<br />Lane 2: HL-60 cell lysate<br />Lane 3: Mouse kidney tissue lysate<br />Lane 4: Mouse lung tissue lysate

    Western blot analysis of TRPV5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SiHa cell lysate
    Lane 2: HL-60 cell lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Mouse lung tissue lysate

  • Western blot analysis of TRPV5 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA720109" style="font-weight: bold;text-decoration: underline;">HA720109</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRPV5 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation