Kallikrein 8 Recombinant Rabbit Monoclonal Antibody [JE62-46]
Catalog# HA720101
Kallikrein 8 Recombinant Rabbit Monoclonal Antibody [JE62-46]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
Kallikrein 8 Recombinant Rabbit Monoclonal Antibody [JE62-46]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Kallikrein 8 aa 161-260/260.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A431 cell lysate, Jurkat cell lysate, Hela cell lysate, human kidney tissue.
Conjugation
unconjugated
Clone Number
JE62-46
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys. Inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.
Background References
1. Herring A.et. al. Genetic knockdown of Klk8 has sex-specific multi-targeted therapeutic effects on Alzheimer\'s pathology in mice. Neuropathol Appl Neurobiol. 2020 Dec
2. Nakazawa H.et. al. Dataset on the effect of knockout of KLK8 in social memory. Data Brief. 2019 Aug
Subcellular Location
Secreted, Cytoplasm.
UNIPROT
Synonyms
Brain serine protease 1 antibody
BSP1 antibody
hK8 antibody
HNP antibody
HPN antibody
Kallikrein 8 (neuropsin/ovasin) antibody
Kallikrein related peptidase 8 antibody
Kallikrein-8 antibody
Kallikrein8 antibody
KLK 8 antibody
ExpandImages
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Western blot analysis of Kallikrein 8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720101, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Predicted band size: 28 kDa
Observed band size: 35 kDa (Glycosylation) -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Kallikrein 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720101, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"