AMSH Recombinant Rabbit Monoclonal Antibody [JE63-41]
Catalog# HA720085
AMSH Recombinant Rabbit Monoclonal Antibody [JE63-41]
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WB
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Human
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unconjugated
Overview
Product Name
AMSH Recombinant Rabbit Monoclonal Antibody [JE63-41]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human AMSH aa 151-200/424.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 48 kDa
Positive Control
MCF-7 cell lysate, 293T cell lysate, HepG2 cell lysate, Hela cell lysate, SW480 cell lysate.
Conjugation
unconjugated
Clone Number
JE63-41
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (By similarity). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
Background References
1. Bednash JS. et. al. The deubiquitinase STAMBP modulates cytokine secretion through the NLRP3 inflammasome. Cell Signal. 2021 Mar
2. Wu F. et. al. Early-onset epilepsy and microcephaly-capillary malformation syndrome caused by a novel STAMBP mutation in a Chinese boy. Mol Med Rep. 2019 Dec
Subcellular Location
Nucleus, Membrane, Cytoplasm, Early endosome.
UNIPROT
Synonyms
AMSH antibody
Associated molecule with the SH3 domain of STAM antibody
Endosome associated ubiquitin isopeptidase antibody
Endosome-associated ubiquitin isopeptidase antibody
MGC126516 antibody
MGC126518 antibody
MICCAP antibody
STABP_HUMAN antibody
STAM binding protein antibody
STAM-binding protein antibody
ExpandImages
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Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: SW480 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 50kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"