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FHL1 Recombinant Rabbit Monoclonal Antibody [JE40-35]

Usd: 385

Specification

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Catalog# HA720075

FHL1 Recombinant Rabbit Monoclonal Antibody [JE40-35]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

FHL1 Recombinant Rabbit Monoclonal Antibody [JE40-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FHL1 aa 6-55/323.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 36 kDa

Positive Control

Human kidney tissue, mouse kidney tissue, rat kidney tissue, Mouse skeletal muscle tissue lysate, Mouse colon tissue lysate, Rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

JE40-35

RRID

AB_3072014

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

Background References

1. Han S. et. al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2020 May

2. Fu Y. et. al. Genome-wide identification of FHL1 as a powerful prognostic candidate and potential therapeutic target in acute myeloid leukaemia. EBioMedicine. 2020 Feb

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q13642 Human

SwissProt: P97447 Mouse

SwissProt: Q9WUH4 Rat

Synonyms

bA535K18.1 antibody

FHL 1 antibody

FHL 1B antibody

FHL-1 antibody

FHL1 antibody

FHL1 protein antibody

FHL1_HUMAN antibody

FHL1A antibody

FHL1B antibody

FLH1A antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-FHL1 antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-FHL1 antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-FHL1 antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-FHL1 antibody (HA720075) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720075) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of FHL1 on different lysates with Rabbit anti-FHL1 antibody (<a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate <br />Lane 2: Mouse colon tissue lysate <br />Lane 3: Rat skeletal muscle tissue lysate <br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720075" style="font-weight: bold;text-decoration: underline;">HA720075</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa

    Western blot analysis of FHL1 on different lysates with Rabbit anti-FHL1 antibody (HA720075) at 1/2,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Mouse colon tissue lysate
    Lane 3: Rat skeletal muscle tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720075, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"