PPM1E Recombinant Rabbit Monoclonal Antibody [JE61-81]
Catalog# HA720063
PPM1E Recombinant Rabbit Monoclonal Antibody [JE61-81]
-
WB
-
Human
-
unconjugated
Overview
Product Name
PPM1E Recombinant Rabbit Monoclonal Antibody [JE61-81]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PPM1E aa 514-563/755.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 84 kDa
Positive Control
293T cell lysates;SH-SY5Y cell lysates
Conjugation
unconjugated
Clone Number
JE61-81
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene.
Background References
1. Ishida A. et. al. Functions and dysfunctions of Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E. Arch Biochem Biophys. 2018 Feb
2. Chen MB. et. al. AMPKalpha phosphatase Ppm1E upregulation in human gastric cancer is required for cell proliferation. Oncotarget. 2017 May
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
CaMKN antibody
CaMKP-N antibody
CaMKP-nucleus antibody
KIAA1072 antibody
Nuclear calmodulin dependent protein kinase phosphatase antibody
Partner of PIX 1 antibody
Partner of PIX-alpha antibody
Partner of PIXA antibody
POPX1 antibody
ExpandImages
-
Western blot analysis of PPM1E on different lysates with Rabbit anti-PPM1E antibody (HA720063) at 1/5,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720063, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 84 kDa
Observed band size: 84 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"