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WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]

Usd: 385

Specification

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Catalog# HA720062

WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human WDR68 aa 199-248/342.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

39 kDa

Positive Control

Hela cell lysate, 293 cell lysate, human prostate tissue, mouse brain tissue, rat epididymis tissue.

Conjugation

unconjugated

Clone Number

JE61-64

RRID

AB_3072001

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. Alternative splicing results in multiple transcript variants. Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. This protein is involved in the pathway protein ubiquitination, which is part of Protein modification. View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Background References

1. Bonano M. et. al. Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis. Gene Expr Patterns. 2018 Dec

2. Yousefelahiyeh M. et. al. DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B. PLoS One. 2018 Nov

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P61962 Human

SwissProt: P61963 Mouse

Entrez Gene: 303602 Rat

Synonyms

AN11 antibody

Dcaf7 antibody

DCAF7_HUMAN antibody

DDB1- and CUL4-associated factor 7 antibody

HAN11 antibody

Human anthocyanin antibody

Petunia antibody

Seven WD repeat protein of the AN11 family 1 antibody

SWAN 1 antibody

WD repeat domain 68 antibody

Expand

Images

  • Western blot analysis of WDR68 on different lysates with Rabbit anti-WDR68 antibody (<a href="/products/HA720062" style="font-weight: bold;text-decoration: underline;">HA720062</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: 293T (Human embryonic kidney cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720062" style="font-weight: bold;text-decoration: underline;">HA720062</a>, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 39 kDa<br />Observed band size: 39 kDa

    Western blot analysis of WDR68 on different lysates with Rabbit anti-WDR68 antibody (HA720062) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: 293T (Human embryonic kidney cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720062, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720062" style="font-weight: bold;text-decoration: underline;">HA720062</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720062" style="font-weight: bold;text-decoration: underline;">HA720062</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"