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CTNNBIP1 Recombinant Rabbit Monoclonal Antibody [JE58-47]

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Specification

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Catalog# HA720041

CTNNBIP1 Recombinant Rabbit Monoclonal Antibody [JE58-47]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CTNNBIP1 Recombinant Rabbit Monoclonal Antibody [JE58-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CTNNBIP1 aa 1-50/81.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 9 kDa

Positive Control

Hep G2 (Human hepatocellular carcinoma cell),U-937 (Human acute monocytic leukemia cell), A431 (Human epidermoid carcinoma skin squamous cell),293T (Human embryonic kidney cell), SW480 (Human colorectal zdenocarcinoma cell),human skin tissue, human Colon cancer tissue, HepG2.

Conjugation

unconjugated

Clone Number

JE58-47

RRID

AB_3071981

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:500-1:1,000

Target

Function

The protein encoded by this gene binds CTNNB1 and prevents interaction between CTNNB1 and TCF family members. The encoded protein is a negative regulator of the Wnt signaling pathway. Two transcript variants encoding the same protein have been found for this gene. ICAT interacts directly with β-catenin and interferes with the Wnt signaling pathway. Specifically, ICAT prevents the interaction of β-catenin with TCF-4 and inhibits β-catenin--TCF-4-mediated transactivation. The negative regulatory effect of ICAT on the Wnt signaling pathway appears to inhibit tumor cell proliferation. ICAT also induces G2 arrest followed by cell death in colorectal tumor cells. The ectopic induction of ICAT inhibits the expression of β3 Tubulin and thus neuronal differentiation in embryonal carcinoma P19 cells. Structural characteristics of ICAT include a three-helix bundle and a C-terminal tail. The gene encoding human ICAT maps to chromosome 1p36.22.

Background References

1. Wang C. et. al. CTNNBIP1 modulates keratinocyte proliferation through promoting the transcription of beta-catenin/TCF complex downstream genes. J Eur Acad Dermatol Venereol. 2020 Jun

2. Chang JM. et. al. The Alteration of CTNNBIP1 in Lung Cancer. Int J Mol Sci. 2019 Nov

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9NSA3 Human

Synonyms

Beta catenin interacting protein 1 antibody

Beta catenin interacting protein ICAT antibody

Beta-catenin-interacting protein 1 antibody

Catenin beta interacting protein 1 antibody

CNBP1_HUMAN antibody

CTNNBIP 1 antibody

CTNNBIP1 antibody

ICAT antibody

Inhibitor of beta catenin and Tcf 4 antibody

Inhibitor of beta-catenin and Tcf-4 antibody

Expand

Images

  • Western blot analysis of CTNNBIP1 on different lysates with Rabbit anti-CTNNBIP1 antibody (<a href="/products/HA720041" style="font-weight: bold;text-decoration: underline;">HA720041</a>) at 1/2,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell)<br />Lane 2: U-937 (Human acute monocytic leukemia cell)<br />Lane 3: A431 (Human epidermoid carcinoma skin squamous cell)<br />Lane 4: 293T (Human embryonic kidney cell)<br />Lane 5: SW480 (Human colorectal zdenocarcinoma cell)<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 4 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720041" style="font-weight: bold;text-decoration: underline;">HA720041</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 9 kDa<br />Observed band size: 9 kDa

    Western blot analysis of CTNNBIP1 on different lysates with Rabbit anti-CTNNBIP1 antibody (HA720041) at 1/2,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell)
    Lane 2: U-937 (Human acute monocytic leukemia cell)
    Lane 3: A431 (Human epidermoid carcinoma skin squamous cell)
    Lane 4: 293T (Human embryonic kidney cell)
    Lane 5: SW480 (Human colorectal zdenocarcinoma cell)

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 4 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720041, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 9 kDa
    Observed band size: 9 kDa

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CTNNBIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720041" style="font-weight: bold;text-decoration: underline;">HA720041</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CTNNBIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720041, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-CTNNBIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720041" style="font-weight: bold;text-decoration: underline;">HA720041</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-CTNNBIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720041, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of CTNNBIP1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA720041" style="font-weight: bold;text-decoration: underline;">HA720041</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CTNNBIP1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720041, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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