HFE Recombinant Rabbit Monoclonal Antibody [JE58-66]
Catalog# HA720039
HFE Recombinant Rabbit Monoclonal Antibody [JE58-66]
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WB
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Human
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unconjugated
Overview
Product Name
HFE Recombinant Rabbit Monoclonal Antibody [JE58-66]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human HFE aa 299-348/348.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa cell lysate, A375 cell lysate, Caco-2 cell lysate.
Conjugation
unconjugated
Clone Number
JE58-66
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
Background References
1. Milman NT. et. al. Diagnosis and Treatment of Genetic HFE-Hemochromatosis: The Danish Aspect. Gastroenterology Res. 2019 Oct
2. Katsarou MS. et. al. Hemochromatosis: Hereditary hemochromatosis and HFE gene. Vitam Horm. 2019
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
dJ221C16.10.1 antibody
Hemochromatosis antibody
Hemochromatosis protein antibody
Hereditary hemochromatosis protein antibody
Hereditary hemochromatosis protein HLA H antibody
HFE 1 antibody
HFE antibody
HFE_HUMAN antibody
HFE1 antibody
HH antibody
ExpandImages
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Western blot analysis of HFE on different lysates with Rabbit anti-HFE antibody (HA720039) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A375 cell lysate
Lane 3: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720039) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"