PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
Catalog# HA720033
PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PDCD7 Recombinant Rabbit Monoclonal Antibody [JE59-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PDCD7 aa 1-100/485.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse stomach tissue lysate.
Conjugation
unconjugated
Clone Number
JE59-06
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
PD-1 (programmed cell death-1 protein) is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. Expression of PD-1 is detected in mouse thymus, and it is induced in stimulated B and T cell lines, where it may play a role in the negative regulation of various immune responses. PD-2 (also known as PDCD2, PDL2 or B7DC) is highly expressed in placenta, heart, pancreas, lung and liver. PD-2 is also expressed in spleen, lymph nodes and thymus. Pdcd-4 is a nuclear protein localized to the nucleus of proliferating cells. ALG-2 (apoptosis-linked gene 2), also designated programmed cell death protein 6, is a Ca2+-binding protein that participates in regulatory events occurring late in the apoptotic program and where several death signals converge. Pdcd-7, also designated programmed cell death protein 7, is a 485 amino acid protein that promotes apoptosis when overexpressed. Human Pdcd-7 and the mouse Pdcd-7 protein, designated as Es18, share 95% homology in the overlapping region.
Background References
1. Abo Elwafa R. et. al. The clinical and prognostic significance of FIS1, SPI1, PDCD7 and Ang2 expression levels in acute myeloid leukemia. Cancer Genet. 2019 Apr
2. Peng SY. et. al. miR-134 targets PDCD7 to reduce E-cadherin expression and enhance oral cancer progression. Int J Cancer. 2018 Dec
Subcellular Location
Nucleus.
Synonyms
Apoptosis-related protein ES18 antibody
ES18 antibody
HES18 antibody
MGC22015 antibody
Pdcd7 antibody
PDCD7_HUMAN antibody
Programmed cell death 7 antibody
Programmed cell death protein 7 antibody
Images
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Western blot analysis of PDCD7 on different lysates with Rabbit anti-PDCD7 antibody (HA720033) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse stomach tissue lysate (30 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720033) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"