Human Kappa light chain Recombinant Rabbit Monoclonal Antibody [JE56-54]
Catalog# HA720014
Human Kappa light chain Recombinant Rabbit Monoclonal Antibody [JE56-54]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
Human Kappa light chain Recombinant Rabbit Monoclonal Antibody [JE56-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Full length native human IgG.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 12/13 kDa
Positive Control
Human plasma lysates, human spleen tissue lysate, human thymus tissue lysate, human plasma tissue lysate, human tonsil tissue, human breast carcinoma tissue.
Conjugation
unconjugated
Clone Number
JE56-54
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
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IHC-P
-
1:50-1:200
Target
Function
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Background References
1. Ma X. et. al. A novel xenograft mouse model for testing approaches targeting human kappa light-chain diseases. Gene Ther. 2019 May
2. van der Kant R. et. al. Adaption of human antibody λ and κ light chain architectures to CDR repertoires. Protein Eng Des Sel. 2019 Dec
Subcellular Location
Cell membrane, Secreted.
Synonyms
Immunoglobulin kappa variable 1D-16
Ig kappa chain V-I region HK146
Ig kappa chain V-I region HK189
Immunoglobulin kappa constant
Ig kappa chain C regionCurated
Ig kappa chain C region AG
Ig kappa chain C region CUM
Ig kappa chain C region EU
Ig kappa chain C region OU
Ig kappa chain C region ROY
ExpandImages
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Western blot analysis of Human Kappa light chain on human plasma lysates with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 12/13 kDa
Observed band size: 25 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720014) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Human Kappa light chain on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720014, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human spleen tissue lysate
Lane 2: Human thymus tissue lysate
Lane 3: Human plasma tissue lysate -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"