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Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]

Usd: 385

Specification

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Catalog# HA720008

Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Sulfite oxidase aa 100-200.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 60 kDa

Positive Control

293T cell lysate, Mouse liver tissue lysate, human kidney tissue, HepG2.

Conjugation

unconjugated

Clone Number

JE56-74

RRID

AB_3071949

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P
human
Tested Tested
Tested Tested
mouse
Tested Tested
Not Recommended
rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:500-1:1,000

Target

Function

Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins.

Background References

1. Nakamura K. et. al. SUOX is negatively associated with multistep carcinogenesis and proliferation in oral squamous cell carcinoma. Med Mol Morphol. 2018 Jun

2. Jin GZ. et. al. SUOX is a promising diagnostic and prognostic biomarker for hepatocellular carcinoma. J Hepatol. 2013 Sep

Subcellular Location

Mitochondrion intermembrane space.

UNIPROT

SwissProt: P51687 Human

SwissProt: Q8R086 Mouse

Synonyms

EC 1.8.3.1 antibody

mitochondrial antibody

Sulfite oxidase antibody

Sulfite oxidase mitochondrial antibody

Sulfite oxidase, mitochondrial precursor antibody

Suox antibody

SUOX_HUMAN antibody

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Sulfite oxidase KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 60 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Sulfite oxidase KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 60 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: Mouse liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: Mouse liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sulfite oxidase antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Sulfite oxidase was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA720008" style="font-weight: bold;text-decoration: underline;">HA720008</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Sulfite oxidase was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720008, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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