StayGold, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody [A2F7]
Catalog# HA710349
StayGold, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody [A2F7]
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WB
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ELISA
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ICC/IF
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IP
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FC
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Species independent
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unconjugated
Overview
Product Name
StayGold, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody [A2F7]
Antibody Type
Alpaca Nanobody
Immunogen
StayGold fusion protein
Product Specificity
StayGold
Species Reactivity
Species independent
Validated Applications
WB, ELISA, ICC/IF, IP, FC
Conjugation
unconjugated
Clone Number
A2F7
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at –20 ℃, (Avoid freeze / thaw cycles)
Storage Buffer
Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Isotype
VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
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WB
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1:5,000-1:20,000
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ELISA
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1:5,000-1:20,000
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ICC/IF
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1:100-1:1,000
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IP
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1-2ug/sample
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FC
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1µg for 10^6 cells
Target
Function
Anti-StayGold, AlpHcAbs® Rabbit antibody is designed for detecting StayGold fusion protein specifically. Anti-StayGold, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-StayGold, AlpHcAbs® Rabbit antibody reacts with the StayGold selectively, no reactivity with other proteins.
Background References
暂无
Images
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Lane1-6: different amount(200ng-100ng-50ng-25ng-12.5-6.25ng) of mBaojin was loaded.
First antibody:Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(HA710349) at 0.1 µg/ml (1:10000)
Secondary antibody：
Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody (HRP) (1:10000) -
The plate was coated with different amounts of StayGold or its variants. 1:10000 dilution of Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody was used as the primary antibody. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH (HRP) was used as the secondary antibody.
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StayGold-Ensconsin transfected COS-7 cells were stained with Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody (1:500) and Goat Anti-Rabbit IgG Antibody(AF647) was the secondary antibody(1:1000). (This picture is kindly provided by Zhang Lab, School of Life Sciences, Westlake University)
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Different amount of reduced StayGold is detected by Anti-StayGold, AlpHcAbs® Rabbit antibody with 1:5000 dilution, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH (HRP) is used as the secondary antibody.
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The plate was coated with different amounts of StayGold or its variants. 1:5000 dilution of Anti-StayGold, AlpHcAbs® Rabbit antibody was used as the detection antibody, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH (HRP) is used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"