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Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody

Usd: 200

Specification

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Catalog# HA710051

Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody

Application

  • WB

  • ELISA

Reactivity

Overview

Product Name

Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody

Antibody Type

Alpaca Nanobody

Immunogen

Recombinant Rabbit IgG

Product Specificity

Rabbit IgG(H+L)

Validated Applications

WB, ELISA

Product Features

Form

Liquid

Concentration

1mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Store at –20 ℃(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20℃

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Isotype

VHH domain of alpaca IgG2b/2c

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • ELISA

  • 1:10000-1:50000

  • WB

  • 1:10000-1:50000

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) is designed for detecting Fc region of rabbit IgG(H+L) specifically. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibodies to rabbit IgG(H+L) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG., High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution.<br /><br />Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)<br />Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs&reg; VHH(HRP) Secondary Antibody (<a href="/products/HA710051" style="font-weight: bold;text-decoration: underline;">HA710051</a>) at 1/10,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution.

    Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)
    Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710051) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"