Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody (HA710049)
Catalog# HA710049
Alpaca Monoclonal to Rabbit IgG(Fab Fragment specific)
Tested for
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WB
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ELISA
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution.
Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)
Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710049) at 1/10,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)
Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710049) at 1/10,000 dilution was used for 1 hour at room temperature.