CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
Catalog# HA601330
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD42b aa 100-350 / 652.
Species Reactivity
Human
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
Human bone marrow tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
13G2-R
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
-
1:1,000
Target
Function
Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.
Background References
1. Ku NK. et. al. Use of CD42b immunohistochemical stain for the detection of Histoplasma. Ann Diagn Pathol. 2018 Feb;32:47-50.
2. Mäyränpää MI. et. al. Improved identification of endothelial erosion by simultaneous detection of endothelial cells (CD31/CD34) and platelets (CD42b). Endothelium. 2007 Mar-Apr;14(2):81-7.
Subcellular Location
Membrane.
UNIPROT
Synonyms
Antigen CD42b alpha antibody
BDPLT1 antibody
BDPLT3 antibody
BSS antibody
CD 42b antibody
CD42b alpha antibody
CD42B antibody
CD42b antigen antibody
DBPLT3 antibody
GLYCOCALICIN antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (HA601330) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601330) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (HA601330) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601330) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
CD42b Mouse Monoclonal Antibody [13G2]
Application: IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
