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ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]

Usd: 350

Specification

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Catalog# HA601305

ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ESD aa 1-182 / 282.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 31 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse colon tissue lysate, mouse stomach tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, rat stomach tissue lysate.

Conjugation

unconjugated

Clone Number

J0-8-R

RRID

AB_3096565

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:20,000

Target

Function

ESD (esterase D) is also known as S-formylglutathione hydrolase and is a 282 amino acid protein that is a member of the esterase D family. ESD is highly expressed in placenta, kidney, liver and erythrocytes, and is localized to the cytoplasm, as well as to cytoplasmic vesicles. The main function of ESD is to detoxify formaldehyde while providing energy. Formaldehyde is oxidized by ADH5 which yields S-formylglutathione. ESD then catalyzes the hydrolysis of S-formylglutathione to the reduced forms of formic acid and glutathione. In addition, ESD hydrolyzes a variety of different neutral ester substrates and can act as a carboxylesterase. ESD may also act as a cysteine hydrolase which is inactivated by thiol alkylating agents. ESD gene polymorphism can lead to reduced enzymatic activity which may cause susceptibility to many conditions, including toxic liver cirrhosis, retinoblastoma, obesity and autism.

Background References

1. Li W et al. Esterase D enhances type I interferon signal transduction to suppress foot-and-mouth disease virus replication. Mol Immunol 75:112-21 (2016).

2. Kaźmierczak M et al. Esterase D and gamma 1 actin level might predict results of induction therapy in patients with acute myeloid leukemia without and with maturation. Med Oncol 30(4):725 (2013).

Subcellular Location

Cytoplasm. Cytoplasmic vesicle.

UNIPROT

SwissProt: P10768 Human

SwissProt: Q9R0P3 Mouse

SwissProt: B0BNE5 Rat

Synonyms

EC 3.1.2.12 antibody

Es-10 antibody

Es10 antibody

ESD antibody

ESTD_HUMAN antibody

Esterase 10 antibody

Esterase D antibody

Esterase D formylglutathione hydrolase antibody

FGH antibody

FGH, included antibody

Expand

Images

  • Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: HepG2 cell lysate<br />Lane 5: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa<br /><br />Exposure time: 1 minute 13 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

    Exposure time: 1 minute 13 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human kidney tissue lysate<br />Lane 2: Mouse kidney tissue lysate<br />Lane 3: Mouse colon tissue lysate<br />Lane 4: Mouse stomach tissue lysate<br />Lane 5: Rat kidney tissue lysate<br />Lane 6: Rat colon tissue lysate<br />Lane 7: Rat stomach tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa<br /><br />Exposure time: 1 minute 18 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/2,000 dilution.

    Lane 1: Human kidney tissue lysate
    Lane 2: Mouse kidney tissue lysate
    Lane 3: Mouse colon tissue lysate
    Lane 4: Mouse stomach tissue lysate
    Lane 5: Rat kidney tissue lysate
    Lane 6: Rat colon tissue lysate
    Lane 7: Rat stomach tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

    Exposure time: 1 minute 18 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/20,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-ESD KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 31 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601305" style="font-weight: bold;text-decoration: underline;">HA601305</a>) at 1/20,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/20,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-ESD KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/20,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"