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Chromogranin A Recombinant Mouse Monoclonal Antibody [D11-2-R]

Usd: 350

Specification

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Catalog# HA601295

Chromogranin A Recombinant Mouse Monoclonal Antibody [D11-2-R]

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Chromogranin A Recombinant Mouse Monoclonal Antibody [D11-2-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Chromogranin-A aa 415-457/457.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 52 kDa

Positive Control

Human atypical carcinoid tissue, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue.

Conjugation

unconjugated

Clone Number

D11-2-R

RRID

AB_3096765

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell IF-Tissue
human
Not Recommended
Tested Tested
Not Recommended
Not Recommended
Tested Tested
mouse
Not Recommended
Tested Tested
Not Recommended
 
 
rat
Not Recommended
Tested Tested
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:15,000

  • IF-Tissue

  • 1:1,000

Target

Function

Chromogranin A or parathyroid secretory protein 1 (gene name CHGA) is a member of the granin family of neuroendocrine secretory proteins. As such, it is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in the pancreas. In humans, chromogranin A protein is encoded by the CHGA gene. Chromogranin A is the precursor to several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). Chromogranin A induces and promotes the generation of secretory granules such as those containing insulin in pancreatic islet beta cells.

Background References

1. Briolat J et al. New antimicrobial activity for the catecholamine release-inhibitory peptide from chromogranin A. Cell Mol Life Sci 62:377-385 (2005).

2. Aung G et al. Catestatin, a neuroendocrine antimicrobial peptide, induces human mast cell migration, degranulation and production of cytokines and chemokines. Immunology 132:527-539 (2011).

Subcellular Location

Secreted. Secretory vesicle lumen.

UNIPROT

SwissProt: P10645 Human

SwissProt: P26339 Mouse

SwissProt: P10354 Rat

Synonyms

beta Granin antibody

betagranin (N-terminal fragment of chromogranin A) antibody

catestatin antibody

CgA antibody

CHG A antibody

Chga antibody

chromofungin antibody

Chromogranin A antibody

Chromogranin A parathyroid secretory protein 1 antibody

Chromogranin A precursor antibody

Expand

Images

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: pancreas<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: pancreas

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

  • Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (HA601295) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601295) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (HA601295) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601295) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Mouse anti-Chromogranin A antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Mouse anti-Chromogranin A antibody (HA601295) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601295) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Chromogranin A antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Chromogranin A antibody (HA601295) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601295) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue (negative) with Mouse anti-Chromogranin A antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601295" style="font-weight: bold;text-decoration: underline;">HA601295</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue (negative) with Mouse anti-Chromogranin A antibody (HA601295) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601295) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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