CD43 Recombinant Mouse Monoclonal Antibody [A2F8-R]
Catalog# HA601286
CD43 Recombinant Mouse Monoclonal Antibody [A2F8-R]
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD43 Recombinant Mouse Monoclonal Antibody [A2F8-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal human CD43.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, U-937, human B lymphocyte tumor tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
A2F8-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:250
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IHC-P
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1:1,000
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IF-Tissue
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1:1,000
Target
Function
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1.
Background References
1. Fay KT. et. al. Increased mortality in CD43-deficient mice during sepsis. PLoS One. 2018 Sep 18;13(9):e0202656.
2. Ma XB. et. al. Coexpression of CD5 and CD43 predicts worse prognosis in diffuse large B-cell lymphoma. Cancer Med. 2018 Sep;7(9):4284-4295.
Subcellular Location
Membrane, microvillus, uropodium; Nucleus, PML body.
UNIPROT
Synonyms
CD 43 antibody
CD43 antibody
CD43 antigen antibody
Galactoglycoprotein antibody
GALGP antibody
GPL 115 antibody
GPL115 antibody
Human gene for sialophorin antibody
Leucocyte sialoglycoprotein antibody
LEUK_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD43 on different lysates with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: U-937 cell lysate
Lane 5: HT-29 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 100-130 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601286) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43 with Mouse anti-CD43 antibody (HA601286) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43 antibody (HA601286) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601286) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601286) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: tonsil
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Application: IF-Tissue
Species: Human
Site: tonsil
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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