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GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]

Usd: 350

Specification

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Catalog# HA601279

GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human GOT1 aa 16-211 / 413.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 46 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, Caco-2 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse heart tissue lysate, rat liver tissue lysate, rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

A2D6-R

RRID

AB_3096581

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Background References

1. Abrego J. et. al. GOT1-mediated anaplerotic glutamine metabolism regulates chronic acidosis stress in pancreatic cancer cells. Cancer Lett. 2017 Aug 1;400:37-46.

2. Hong C. et. al. Inhibition of GOT1 sensitizes colorectal cancer cells to 5-fluorouracil. Cancer Chemother Pharmacol. 2017 Apr;79(4):835-840.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P17174 Human

SwissProt: P05201 Mouse

SwissProt: P13221 Rat

Synonyms

AATC_HUMAN antibody

Aspartate aminotransferase 1 antibody

Aspartate aminotransferase antibody

Aspartate aminotransferase cytoplasmic antibody

Aspartate aminotransferase cytosolic antibody

ASTQTL1 antibody

cAspAT antibody

cCAT antibody

cysteine aminotransferase, cytoplasmic antibody

cysteine transaminase, cytoplasmic antibody

Expand

Images

  • Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (<a href="/products/HA601279" style="font-weight: bold;text-decoration: underline;">HA601279</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: HL-60 cell lysate (20 µg/Lane)<br />Lane 4: Caco-2 cell lysate (20 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br />Lane 6: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 7: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 8: Rat liver tissue lysate (40 µg/Lane)<br />Lane 9: Rat heart tissue lysate (40 µg/Lane)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 1 minute 41 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601279" style="font-weight: bold;text-decoration: underline;">HA601279</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: HL-60 cell lysate (20 µg/Lane)
    Lane 4: Caco-2 cell lysate (20 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)
    Lane 6: Mouse liver tissue lysate (40 µg/Lane)
    Lane 7: Mouse heart tissue lysate (40 µg/Lane)
    Lane 8: Rat liver tissue lysate (40 µg/Lane)
    Lane 9: Rat heart tissue lysate (40 µg/Lane)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 38 kDa

    Exposure time: 1 minute 41 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (<a href="/products/HA601279" style="font-weight: bold;text-decoration: underline;">HA601279</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-GOT1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601279" style="font-weight: bold;text-decoration: underline;">HA601279</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-GOT1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"