IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R]
Catalog# HA601271
IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IREB2 aa 1-963.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 105 kDa
Positive Control
Jurkat cell lysate, A549 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse heart tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
9D1-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Background References
1. Samaniego F. et. al. Molecular characterization of a second iron-responsive element binding protein, iron regulatory protein 2. Structure, function, and post-translational regulation. J. Biol. Chem. 269:30904-30910(1994).
Subcellular Location
Cytoplasm.
Synonyms
ACO3 antibody
FLJ23381 antibody
IRE BP2 antibody
IRE-BP 2 antibody
IREB 2 antibody
IREB2 antibody
IREB2_HUMAN antibody
Iron regulatory protein 2 antibody
iron responsive element binding protein 2 antibody
Iron-responsive element-binding protein 2 antibody
ExpandImages
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Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (HA601271) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601271) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"