NAPSIN A Recombinant Mouse Monoclonal Antibody [A9B12-R]
Catalog# HA601242
NAPSIN A Recombinant Mouse Monoclonal Antibody [A9B12-R]
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IHC-P
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Human
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unconjugated
Overview
Product Name
NAPSIN A Recombinant Mouse Monoclonal Antibody [A9B12-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human NAPSIN A aa 51-200 / 420.
Species Reactivity
Human
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
Human kidney tissue, human lung cancer tissue.
Conjugation
unconjugated
Clone Number
A9B12-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
Target
Function
Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. The name napsin comes from novel aspartic proteinase of the pepsin family. The activation peptide of an aspartic proteinase acts as an inhibitor of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. Detection of NAPSA gene expression can be used to distinguish adenocarcinomas from other forms of lung cancer.
Background References
1. Weidemann S et al. Napsin A Expression in Human Tumors and Normal Tissues. Pathol Oncol Res. 2021 Apr
2. Wu J et al. Napsin A Expression in Subtypes of Thyroid Tumors: Comparison with Lung Adenocarcinomas. Endocr Pathol. 2020 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
Asp 4 antibody
ASP4 antibody
Aspartyl protease 4 antibody
KAP antibody
Kdap antibody
Kidney derived aspartic protease like protein antibody
NAP1 antibody
NAPA antibody
Napsa antibody
NAPSA_HUMAN antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-NAPSIN A antibody (HA601242) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601242) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-NAPSIN A antibody (HA601242) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601242) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human colon tissue (negative) with Mouse anti-NAPSIN A antibody (HA601242) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601242) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"