Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]
Catalog# HA601226
Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]
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WB
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Human
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unconjugated
Overview
Product Name
Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human ORM1 aa 1-201 / 201.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, human liver tissue lysate.
Conjugation
unconjugated
Clone Number
A4-G2-A3-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Background References
1. Fitos I et al. Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein. Biochim Biophys Acta 1760:1704-1712(2006).
2. Zsila F et al. The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra. Biochim Biophys Acta 1770:797-809(2007).
Subcellular Location
Secreted.
UNIPROT
Synonyms
A1AG1_HUMAN antibody
AGP 1 antibody
AGP A antibody
AGP antibody
AGP1 antibody
Alpha 1 acid glycoprotein antibody
Alpha-1-acid glycoprotein 1 antibody
alpha-1-AGP antibody
Epididymis secretory sperm binding protein Li 153w antibody
glycoprotein, alpha-1-acid, of serum antibody
ExpandImages
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Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (HA601226) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 37/45 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601226) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"