BrdU Recombinant Antibody [PSH0-18] - Mouse IgG1 (Chimeric)
Catalog# HA601222
BrdU Recombinant Antibody [PSH0-18] - Mouse IgG1 (Chimeric)
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IHC-P
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IF-Cell
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IF-Tissue
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Species independent
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unconjugated
Overview
Product Name
BrdU Recombinant Antibody [PSH0-18] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
BrdU-OVA
Product Specificity
This Mouse Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (HA721283). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
Species Reactivity
Species independent
Validated Applications
IHC-P, IF-Cell, IF-Tissue
Positive Control
BrdU treated mouse embryo brain tissue, BrdU treated mouse embryo cartilage tissue, BrdU treated mouse embryo liver tissue, BrdU treated NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH0-18
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:10,000
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IF-Cell
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1:50
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IF-Tissue
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1:1,000
Target
Function
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues and has been studied as a radiosensitizer and diagnostic tool in people with cancer. During S phase of the cell cycle (when DNA replication occurs), BrdU can be incorporated in place of thymidine in newly synthesized DNA molecules of dividing cells. Cells that have recently performed DNA replication or DNA repair can be detected with antibodies specific for BrdU using techniques such as immunohistochemistry or immunofluorescence. BrdU-labelled cells in humans can be detected up to two years after BrdU infusion. Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard. However, because it is neither radioactive nor myelotoxic at labeling concentrations, it is widely preferred for in vivo studies of cancer cell proliferation. However, at radiosensitizing concentrations, BrdU becomes myelosuppressive, thus limiting its use for radiosensitizing. BrdU differs from thymidine in that BrdU substitutes a bromine atom for thymidine's CH3 group. The Br substitution can be used in X-ray diffraction experiments in crystals containing either DNA or RNA. The Br atom acts as an anomalous scatterer and its larger size will affect the crystal's X-ray diffraction enough to detect isomorphous differences as well. Bromodeoxyuridine releases gene silencing caused by DNA methylation. DNA with BrdU transcribes as usual DNA, with guanine included into RNA as a complement to BrdU.
Background References
1. Wang T et al. Unexpected BrdU inhibition on astrocyte-to-neuron conversion. Neural Regen Res. 2022 Jul
2. Yu J et al. BrdU Incorporation Assay to Analyze the Entry into S Phase. Methods Mol Biol. 2022
Subcellular Location
Nucleus.
Synonyms
Bromodeoxyuridine antibody
BUdr antibody
Images
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Immunohistochemical analysis of paraffin-embedded mouse embryo brain tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryo liver tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of NIH/3T3 cells (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/50 dilution.
Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCL in TBST for 30 minutes at room temperature. permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-BrdU antibody (HA601222) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of paraffin-embedded mouse embryo brain tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601222, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse embryo cartilage tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601222, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"