MLH1 Mouse Monoclonal Antibody [A10A3]
Catalog# HA601184
MLH1 Mouse Monoclonal Antibody [A10A3]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
MLH1 Mouse Monoclonal Antibody [A10A3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human MLH1 aa 301-600 / 756.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 85 kDa
Positive Control
A431 cell lysate, HeLa cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human appendix tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
A10A3
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
1mg/mL(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Background References
1. Yue Q et al. Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. Adv Sci (Weinh). 2024 May
2. Guan J et al. MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway. Cancer Cell. 2021 Jan
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
COCA 2 antibody
COCA2 antibody
DNA mismatch repair protein Mlh1 antibody
FCC 2 antibody
FCC2 antibody
hMLH 1 antibody
hMLH1 antibody
HNPCC 2 antibody
HNPCC antibody
HNPCC2 antibody
ExpandImages
-
Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Daudi cell lysate
Lane 4: K-562 cell lysate
Lane 5: HL-60 cell lysate
Lane 6: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84.6 kDa
Observed band size: 85 kDa
Exposure time: 1 minutes 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601184) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
MLH1 Recombinant Mouse Monoclonal Antibody [PSH20-67]
Application: WB,IHC-P,IF-Cell
Reactivity: Human
Conjugate: unconjugated
MLH1 Recombinant Rabbit Monoclonal Antibody [PSH01-74]
Application: IHC-P,IF-Tissue,IF-Cell
Reactivity: Human
Conjugate: unconjugated
MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
Application: IHC-P,IF-Cell,WB
Reactivity: Human
Conjugate: unconjugated
MLH1 Recombinant Rabbit Monoclonal Antibody [PD01-61]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
