DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R]
Catalog# HA601162
DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human DAP Kinase 1 aa 451-700 / 1,430.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 160 kDa
Positive Control
A549 cell lysate, TF-1 cell lysate, HepG2 cell lysate, human brain tissue lysate, human lung carcinoma tissue.
Conjugation
unconjugated
Clone Number
12B2-R
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IHC-P
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1:2,000
Target
Function
Death-associated protein kinase 1 is an enzyme that in humans is encoded by the DAPK1 gene. Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. In melanocytic cells DAPK1 gene expression may be regulated by MITF. Depletion of DAPK1 results in inhibition of tumor cell count and volume growth in cellular and animal models of triple receptor-negative breast cancer, from individuals with p53-mutant cancers. This has not been demonstrated in actual patients.
Background References
1. Li T et al. Dapk1 improves inflammation, oxidative stress and autophagy in LPS-induced acute lung injury via p38MAPK/NF-κB signaling pathway. Mol Immunol. 2020 Apr
2. Movahhed P et al. The impact of DAPK1 and mTORC1 signaling association on autophagy in cancer. Mol Biol Rep. 2022 Jun
Subcellular Location
Cytoplasm, cytoskeleton.
UNIPROT
Synonyms
DAK1 antibody
DAP K1 antibody
DAP kinase 1 antibody
DAPK 1 antibody
DAPK antibody
DAPK1 antibody
DAPK1_HUMAN antibody
Death Associated Protein Kinase 1 antibody
Death-associated protein kinase 1 antibody
DKFZp781I035 antibody
Images
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Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution.
Lane 1: A549 cell lysate (30 µg/Lane)
Lane 2: TF-1 cell lysate (30 µg/Lane)
Lane 3: HepG2 cell lysate (30 µg/Lane)
Lane 4: Human brain tissue lysate (30 µg/Lane)
Predicted band size: 160 kDa
Observed band size: 160 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601162) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601162) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
DAP Kinase 1 Mouse Monoclonal Antibody [12B2]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
